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Biology 230

Polymerase Chain Reaction

(BIOL 230, Lab Experiment 20)

BIOL 230 lab contents BIOL 230 My home page
 

This page is designed to help you interpret your experimental results.
Try a real application of PCR to diagnose a disease.
Do you need to review Hardy-Weinberg?

BioScience article | Tu data | Th data

About quaggas.

  

Samples from three different students are in lanes A, B, and C. The size ladder in D is in 100 bp increments (100, 200, 300, etc.)

Which student sample is homozygous for alu 550 bp? For alu 850 bp? Heterozygous for the two alu alleles?
*Primer-dimer artifacts: the primers can sometimes anneal to themselves and create small templates for PCR amplification - these are the so-called primer-dimer artifacts.

 

The following data are for alu alleles: A and B. You can be homozygous for 200, homozygous for 500, or heterozygous. There are two alleles for gene A: 200 bp and 500 bp; and for gene B: 100 bp and 400 bp.

Class Data 2006

Genotype

550/550

550/850

850/850

Number

9

5

5
 

p2

2pq

q2

Actual

0.47

0.26

0.26

Expected

0.47

0.25

0.31

1. Do you think Alu is useful for distinguishing people in this class? Not using just these two genotypes.

2. Would it matter if the two samples came from people who were closely related? Related individuals are more likely to have similar genes.

3. If the two genotypes were the same would that prove that the samples came from the same person? Not using just these two genotypes.

4. If the PIC for the selected population and the Hardy-Weinberg population are different. What is the significance of this? Hardy is based on a static population. What are the assumptions for a Hardy-Weinberg population? Hint