A.	B.	C.
Locate some cell walls in the photos. Which photograph shows the plant tissue before digestion with cellulase? Which photograph shows protoplasts? How can you tell? What shape are the protoplasts? Why?
Below, protoplast fusion.

Agrobacterium can transform plants by inserting its Ti plasmid into the plant cells. The Ti plasmid contains T (tumor-inducing) DNA which integrates into the genome of an infected plant. The T-DNA stimulates local cellular growth (the crown gall) and simultaneously causes the production of certain products used by the bacteria as a source of nutritional carbon and nitrogen.

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