| Background
Your project will be associated with one of five "companies:"
Pond Scum
The greenest fuel may be provided by little green cells.
More than 100,000 strains of algae have been identified. Many of
them grow rapidly and can be cultivated to develop high oil content,
often more than 60 percent of their biomass. Basic production requirements
are simple: water—even saline water—and carbon dioxide.
Current research efforts are focused on maximizing oil production.
Culture one or more unicellular algae to
Questions:
-Compare cell growth with oil production under different conditions
[e.g., light, pH, flat flask, shaker; nutrients (treated sewage,
change N, other chemicals, saltwater]. How can you get the most
fuel? Growth | Oil
staining.pdf
La’au
The use of higher plants in the treatment of disease is as old as
the art of healing itself. Isaiah of Biblical times suggested that
a plaster of figs be placed on boils as a treatment of this form
of infection. The Chinese have used plants and herbs for centuries
in the treatment of many diseases. Thousands of phytochemicals have
been isolated and several serve as plant defenses against microbial
infection (3). Preliminary studies showed that plants have antibacterial
properties but these studies were done over 60 years ago (2). The
idea of using plants was abandoned with the discovery of antibiotics
from soil bacteria. The widespread resistance of bacterial pathogens
to conventional antibiotics has prompted renewed interest in the
use of alternative natural microbial inhibitors such as amtimicrobial
peptides (AMPs). AMPs, termed bacteriocins, are ribosomally synthesised
polypeptides, which have bactericidal or bacteriostatic effect.
A variety of antimicrobial peptides have been discovered in recent
years including defensins, found in human skin, magainin, found
in frog skin, and squalamine, from the spiny dogfish shark.. Additionally,
the rise in foodborne infections has prompted renewed interest in
the use of alternative natural microbial inhibitors (4)
Possible
test organisms:
Questions:
-Effects on bacterial/fungal growth of sublethal concentration(s)
of an extract of plant parts (berries, leaves, etc) or insect
parts.
-Determine the Minimal inhibitory concentration (MIC)
and/or minimal lethal concentration (MLC) of an extract.
-The presence of lysozyme against gram-positive cells. Gram-negative
cells.
-Does antimicrobial activity increase if you infect or vaccinate?
-What is your control?
PharMar
There is a high rate of rediscovery of antibiotic compounds in terrestrial
sources, which means the rate of novel compound discovery has been
going down. The sea’s potential as a resource is largely untapped.
So far, there are only four marine-based drugs on the market and
about 40 in clinical/preclinical development. A major therapeutic
area for marine natural products is infection. Novel antimicrobial
peptides (AMPs) have been isolated from the blood of the spider
crab Hyas araneus and the green sea urchin Strongylocentrotus
droebachiensis (1). The widespread resistance of bacterial
pathogens to conventional antibiotics has prompted renewed interest
in the use of alternative natural microbial inhibitors such as AMPs.
AMPs, termed bacteriocins, are ribosomally synthesised polypeptides,
which have bactericidal or bacteriostatic effect. A variety of antimicrobial
peptides have been discovered in recent years including defensins,
found in human skin, magainin, found in frog skin, and squalamine,
from the spiny dogfish shark.
Questions:
-Effects on growth of sublethal concentration(s) of an extract
of red or brown algae.
-Determine the Minimal inhibitory concentration (MIC) and/or minimal
lethal concentration (MLC) of an extract.
Gaia
The Gaia hypothesis proposes that living and nonliving
parts of the earth are viewed as a complex interacting system. Named
after the Greek earth goddess, this hypothesis proposes that all
living things have a regulatory effect on the Earth's environment
that promotes life overall.
Questions:
-Evaluate indoor air quality in
Pacific Heights.
-Determine the source of fecal contamination (fecal
coliforms, enterococci) in a local creek (map).
-Determine the effect of environmental change on spring productivity
(read more…)
-Effect of triclosan on an aquatic food chain (algae-protozoa-bacteria).
Growth
| Purpose
Working in groups of two students, you will
1. Answer the following questions in the background:
-What will you be looking for?
-Why is this important?
2. What will you look for with your experiments?
-How many trials will you do?
3. Discuss the significance of your results
4. Write your report in the proper
format.
Hypotheses
State your hypothesis
(& alternative hypotheses)
Materials Available
You must turn in the Supply Request
form before you begin working.
Cultures available:
Aspergillus sp.
Escherichia coli
Micrococcus luteus
Salmonella enterica
Shigella sonnei
Staphylococcus aureus
Saccharomyces cerevisiae
Penicillium sp.
Pseudomonas aeruginosa
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus mutans
Procedures at Qualitas, La'au
and PharMar
• Sample preparation. Prepare an extract by grinding
the appropriate tissue in a few milliliters of solvent in
a mortar and pestle. Possible solvents are sterile water;
methyl alcohol, ethyl alcohol, and acetone.
• Screening
• Serial dilutions
* MIC
• Lysozyme
* Bacteriocins
(AMPs)
• Isolating active compounds
• Bacterial numbers
• Population growth
|
Results
Displaying data
Discussion & Conclusions
Literature Cited
You are encouraged to read all the available information on
the topic for your paper. To select appropriate references,
you must evaluate your sources of information. Print and electronic
encyclopedias are often good to get familiar with a topic:
however, these are not appropriate references for
a research paper. The most reliable information is that published
in peer-reviewed journals. Peer-reviewed means that the author's
work has been read and accepted by colleagues in his/her discipline.
Start your literature search with Highwire, PubMed, or another
science database and search on your topic. Read the abstracts
and keep a list of interesting articles. Note, you must read
and cite the articles. Highwire, PubMed, and other databases
are not citations; these are indexes to articles.
1. Aldridge, S. “Marine Bioprospecting for Novel Drug.”
Genetic Engineering News 26(21):00-00, Dec 1 2006.
2. Carlson, J. J., H. G. Douglas, and J. Robertson. 1948.
“Antibacterial substances separated from plants.”
Journal of Bacteriology 55(2):241-248.
3. Cowan, M. M. 1999. “Plant Products as Antimicrobial
Agents.” Clinical Microbiology Reviews 12(4);
564-582. pdf
4.Friedman M, Henika PR, Levin CE, Mandrell RE. 2004 “Antibacterial
activities of plant essential oils and their components against
Escherichia coli O157:H7 and Salmonella enterica
in apple juice.” Journal of Agricultural Food Chemistry
52(19):6042-6048.
5. Levy, S. R. "The challenge of antibiotic resistance."
Scientific American March 1998 pp. 48-53. |
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