Industrial Microbiology
Industrial Microbiology Study Questions.
Read Chapters 5, 6, and 28 if you need to review.
1. Assume that you use the DNA polymerase from
Thermus aquaticus
to make copies of frog DNA. The DNA that results will be
a. Frog DNA.
b.
T. aquaticus
DNA.
c. Frog x
Thermus
hybrid.
d.
E. coli
DNA.
e. None of the above.
2. Which of the following food additives is not produced by microorganisms?
a. Amylase.
b. Citric acid.
c. Sodium nitrate.
d. Glutamic acid.
e. Invertase.
3. Wine is made from fruit juices by
a. The addition of alcohol.
b. Bacterial production of carbon dioxide.
c. The addition of sugar.
d. Anaerobic fungal growth.
e. Malting.
4. Which of the following is an undesirable contaminant in winemaking?
a.
Acetobacter
.
b. Lactic acid bacteria.
c.
Clostridium
.
d.
Bacillus
.
e.
Saccharomyces
.
5. Which reaction leads to all of the others?
a. Sucrose -> ethanol.
b. Ethanol -> acetic acid.
c. Malic acid -> lactic acid.
d. CO
2
-> sucrose.
e. None of the above.
6. When compared to a flask culture, a bioreactor offers all of the following advantages except
a. Larger culture volumes can be grown.
b. Instrumentation for monitoring environmental conditions.
c. Uniform aeration and mixing.
d. Aseptic sampling.
e. None of the above.
7. Microbial products can be improved by
a. Isolating new strains.
b. Mutating existing strains.
c. Genetically engineering strains.
d. Modifying culture conditions.
e. All of the above.
8. Bioremediation is usually done by
a. Genetically-engineered superbugs.
b. Genetically engineered bacteria.
c. Natural microbes already in the polluted location.
d. Trucking polluted soil/water to a dump.
e. None of the above, it can't be done.
9. Cellulase attached to a membrane filter will
a. Degrade cellulose.
b. Degrade the membrane.
c. Do nothing, it requires a cell.
d. Degrade lactose.
e. None of the above.
10. All of the following are industrial products produced by microbes except
a. Amino acids in food supplements.
b. Antibiotics.
c. Industrial enzymes.
d. Uranium
e. Vitamin B12 and riboflavin.
11. Methane made from biomass is produced by
a. Anaerobic respiration.
b. Fermentation.
c. The Krebs cycle.
d. Oxidation.
e. None of the above.
12. Which of the following pairs is mismatched?
a.
Propionibacterium
-- Swiss cheese.
b.
Penicillium
-- blue cheese.
c.
Streptococcus
-- yogurt.
d.
Acetobacter
-- vinegar.
e.
Bacillus
-- hard cheese.
13. Grape juice before fermentation is called must. What will be produced if must is aerated?
a. Ethyl alcohol + CO
2
.
b. CO
2
+ H
2
O.
c. Methane.
d. Acetic acid.
e. None of the above.
14. You are growing
Bacillus subtilis
in a bioreactor and notice that the growth rate has slowed and the pH has decreased. You suspect the bacteria are
a. Using the Krebs cycle.
b. Fermenting.
c. Photosynthesizing.
d. Using proteins.
e. None of the above.
15. Which of the following is not an agricultural product made by genetic engineering?
a. Frost retardant.
b. Insecticide.
c. Glyphosate resistance.
d. Drought resistance.
e. Nitrogenase (nitrogen fixation).
16. Which of the following is not a disadvantage of
E. coli
for making a human gene product?
a. Endotoxin may be in the product.
b. It doesn't secrete most proteins.
c. Its genes are well known.
d. It can't process introns.
e. None of the above.
17. A technique used to identify bacteria carrying a specific gene is
a. Transformation.
b. Southern blotting.
c. Cloning.
d. Transcription.
e. None of the above.
18. Which of the following is mismatched?
a. Southern blot - DNA.
b. Northern blot - RNA.
c. Western blot - protein.
d. Colony blot - DNA.
e. Eastern blot - enzymes.
19. Foreign DNA can be inserted into cells by all of the following except
a. Transformation.
b. Electroporation.
c. Protoplast fusion.
d. A gene gun.
e. Electrophoresis.
20. Which of the following is the best definition of fermentation?
a. The reduction of glucose to pyruvic acid.
b. The oxidation of glucose with organic molecules serving as electron acceptors.
c. The complete catabolism of glucose to carbon dioxide and water.
d. The production of energy by substrate-level phosphorylation.
e. The production of ethyl alcohol from glucose.